The original DYKDDDDK peptide (the same epitope recognized by Sigma's Anti-FLAG® **antibodies) was an 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7 fused at the amino-terminus of GAL4 (yeast transcription factor).
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This antibody is optimally suited for monitoring the expression of FLAG™ tagged fusion proteins. As such, this antibody can be used to identify fusion proteins containing the FLAG™ epitope. The antibody recognizes the epitope tag fused to the amino- and carboxy- termini of targeted proteins.
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The FLAG ® system is a popular epitope tagging system for recombinant protein technology. The FLAG technology is comprised of a small 8 amino acid hydrophilic epitope tag (DYKDDDDK) and the corresponding monoclonal antibody(s). Monoclonal antibodies for research use are typically puriﬁ ed on either protein A or protein G afﬁ nity resin. We recently employed a proprietary, highly speciﬁc ...
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It totally depends on the buffer you use. the M2 Flag sigma beads cant take SDS or Na-deoxycholate, whereas Dynabeads can. I did indeed compare them and observed the pulldown is highly depended on...
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Apr 27, 2021 · Subsequently, the cells were washed with 1 × PBS and subjected for RT-qPCR analysis with the primers listed in Table S3 and western blot assay with the FTO antibody (Abcam), c-Jun antibody (Cell signaling technology, 9165S), C/EBPβ antibody (Abcam), JUNB antibody (Cell signaling technology), FLAG antibody (TransGen biotech), β-Tubulin ...
Replication-dependent histones (RDH) are required for packaging of newly synthetized DNA into nucleosomes during the S phase when their expression is highly upregulated. However, the mechanisms of ...
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Apr 27, 2021 · ANTI-FLAG ® M2 Affinity Agarose Gel and aphidicolin were purchased from Sigma-Aldrich. SYBR–Gold was purchased from Molecular Probes. SYBR–Gold was purchased from Molecular Probes. All reagents were of the analytical reagent grades and utilized as received.
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ANTI-FLAG M2 Dilute ANTI-FLAG M2 antibody from 0.1 to 0.5 µg/ml. Use TBS with antibody concen- 3% non-fat dry milk as diluent. tration is too high Secondary antibody Sigma recommends initial dilutions of 1:10,000 for ECL and 1:80,000 concentration is too for ECL+.
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#M20018 Anti-DYKDDDDK-Tag Mouse Antibody (Agarose Conjugated) (Same as Sigma’s Anti-FLAG M2) #M20118 Anti-DYKDDDDK-Tag Mouse Antibody (Magnetic Beads) (Same as Sigma’s Anti-FLAG M2) Western blot analysis of extracts from 3T3 cells, using SYTL4 antibody.
200 [micro]l of the quantified lysates was incubated with 1 fig of mouse anti-Flag M2 (Sigma-Aldrich, F1804) or anti-HA tag (C29F4) antibody (Cell Signaling T... acronyms.thefreedictionary... Captopril price,buy Captopril - chemicalbook
ANTI-FLAG(R) M2-Cy3(TM) antibody produced in mouse human, flag® (Sigma-Aldrich Cat# A9594, RRID:AB_439700) monoclonal antibody. PMID:29549340, PMID:31433293, PMID ...
The anti-human polyclonal capture reagents are some of the most robust reagents I have in my biophysical and biochemical studies so far. The capture is very stable for a long time (hours) in an experiment, the anti-human coated chips/plates can be re-used multiple times (sometimes for weeks to a month) without losing antibody capturing capacity and chip surface integrity.
FLAG ® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only. The Life Science industry has been in the grips of a reproducibility crisis for a number of years.