The antibody recognizes the DYKDDDDK peptide (the same epitope recognized by Sigma's Anti-FLAG ® antibodies) fused to either the amino or carboxy terminus of targeted proteins. The binding specificity of this antibody is NOT dependent on the presence of divalent metal cations.
The original DYKDDDDK peptide (the same epitope recognized by Sigma's Anti-FLAG® **antibodies) was an 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7 fused at the amino-terminus of GAL4 (yeast transcription factor).

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This antibody is optimally suited for monitoring the expression of FLAG™ tagged fusion proteins. As such, this antibody can be used to identify fusion proteins containing the FLAG™ epitope. The antibody recognizes the epitope tag fused to the amino- and carboxy- termini of targeted proteins.
This antibody is optimally suited for monitoring the expression of FLAG™ tagged fusion proteins. As such, this antibody can be used to identify fusion proteins containing the FLAG™ epitope. The antibody recognizes the epitope tag fused to the amino- and carboxy- termini of targeted proteins.

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(Binds to same epitope as Sigma's Anti-FLAG ... (CSL) protein using Myc-Tag (9B11) ... -activated Sepharose ® beads. Coupling protein to CNBr activated Sepharose Beads.
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3 reviews. Compare Anti-FLAG Flow Cytometry Antibody Products from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and more.
The FLAG ® system is a popular epitope tagging system for recombinant protein technology. The FLAG technology is comprised of a small 8 amino acid hydrophilic epitope tag (DYKDDDDK) and the corresponding monoclonal antibody(s). Monoclonal antibodies for research use are typically purifi ed on either protein A or protein G affi nity resin. We recently employed a proprietary, highly specific ...

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For flag tag you might try anti-flagM2 from sigma. Good luck!!! karmen. abcam has very good c-myc Ab but very expensive-Katja W.-i agree with carmen for flag antibody. agarose m2 is very good in my hands and ver reproductive-fred_33-QUOTE (Baoxiu @ Feb 13 2007, 07:51 AM)
It totally depends on the buffer you use. the M2 Flag sigma beads cant take SDS or Na-deoxycholate, whereas Dynabeads can. I did indeed compare them and observed the pulldown is highly depended on...

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FLAG ® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only. This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility
Apr 27, 2021 · Subsequently, the cells were washed with 1 × PBS and subjected for RT-qPCR analysis with the primers listed in Table S3 and western blot assay with the FTO antibody (Abcam), c-Jun antibody (Cell signaling technology, 9165S), C/EBPβ antibody (Abcam), JUNB antibody (Cell signaling technology), FLAG antibody (TransGen biotech), β-Tubulin ...

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Flag-RXRα/385, GFP-Nur77/Δ2, and Bcl-2 were transfected into HEK293T cells as indicated. HM and cytosolic fractions were prepared and levels of Flag-RXRα/385, GFP-Nur77/Δ2, and Bcl-2 were determined by immunoblotting with anti-Flag (Sigma), anti-GFP, and anti-Bcl-2 antibody, respectively.
Replication-dependent histones (RDH) are required for packaging of newly synthetized DNA into nucleosomes during the S phase when their expression is highly upregulated. However, the mechanisms of ...

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Immunohistochemistry for M1Flag Antibody (Sigma, Cat# F3040) (alcohol fixed paraffin embedded bone sections) Trieu Nguyen and Ed Hsiao, Conklin lab. R. 20071105 REAGENTS VECTOR M.O.M. Immunodetection Kit (Peroxidase) (Vector, Cat# PK‐2200) WORKING SOLUTIONS: M.O.M. Mouse Ig Blocking Reagent: 2 drops of stock solution to 2.5ml TBS
Apr 27, 2021 · ANTI-FLAG ® M2 Affinity Agarose Gel and aphidicolin were purchased from Sigma-Aldrich. SYBR–Gold was purchased from Molecular Probes. SYBR–Gold was purchased from Molecular Probes. All reagents were of the analytical reagent grades and utilized as received.

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ANTI-FLAG M2 Dilute ANTI-FLAG M2 antibody from 0.1 to 0.5 µg/ml. Use TBS with antibody concen- 3% non-fat dry milk as diluent. tration is too high Secondary antibody Sigma recommends initial dilutions of 1:10,000 for ECL and 1:80,000 concentration is too for ECL+.

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#M20018 Anti-DYKDDDDK-Tag Mouse Antibody (Agarose Conjugated) (Same as Sigma’s Anti-FLAG M2) #M20118 Anti-DYKDDDDK-Tag Mouse Antibody (Magnetic Beads) (Same as Sigma’s Anti-FLAG M2) Western blot analysis of extracts from 3T3 cells, using SYTL4 antibody.
200 [micro]l of the quantified lysates was incubated with 1 fig of mouse anti-Flag M2 (Sigma-Aldrich, F1804) or anti-HA tag (C29F4) antibody (Cell Signaling T... acronyms.thefreedictionary... Captopril price,buy Captopril - chemicalbook
ANTI-FLAG(R) M2-Cy3(TM) antibody produced in mouse human, flag® (Sigma-Aldrich Cat# A9594, RRID:AB_439700) monoclonal antibody. PMID:29549340, PMID:31433293, PMID ...
The anti-human polyclonal capture reagents are some of the most robust reagents I have in my biophysical and biochemical studies so far. The capture is very stable for a long time (hours) in an experiment, the anti-human coated chips/plates can be re-used multiple times (sometimes for weeks to a month) without losing antibody capturing capacity and chip surface integrity.
FLAG ® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only. The Life Science industry has been in the grips of a reproducibility crisis for a number of years.

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